phosphor akt Search Results


phosphor-akt1 thr308 antibody  (WuXi AppTec)
90
WuXi AppTec phosphor-akt1 thr308 antibody
The <t>phosphorylated-AKT1</t> <t>Thr308</t> level is relatively higher in Shock-Ens, while the expression of PLCβ3 is relatively higher in Mor-Ens. (A) Left, schematic of the experimental procedure for the in-situ proximity ligation assay. Right, the working principal diagram of PLA. (B, D) Representative images of PLA signaling for phosphor-AKT1 Thr308 or PLCβ3 in the VTA. Green: EGFP; Red: p-AKT1 or PLCβ3. Scale bar: left 100 μm, right 25 μm. Dashed lines: outline of EGFP + cell. (C, E) The violin plot and cumulative probability curves illustrate the distribution of the PLA signal intensity in each EGFP + cell. [Mann-Whitney test: (C) Left, Shock-Ens n = 822 cells from 5 mice, Mor-Ens n = 754 cells from 5 mice, U = 240371, P < 0.001; (E) left, Shock-Ens n = 1340 cells from 8 mice, Mor-Ens n = 1415 cells from 8 mice, U = 898116, P = 0.017. Kolmogorov-Smirnov test: (C) right, D = 0.179, P < 0.001; (E) right, D = 0.056, P = 0.026]. *P < 0.05, **P < 0.01, ***P < 0.001. Data are shown as mean ± SEM.
Phosphor Akt1 Thr308 Antibody, supplied by WuXi AppTec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phosphor-akt1 thr308 antibody/product/WuXi AppTec
Average 90 stars, based on 1 article reviews
phosphor-akt1 thr308 antibody - by Bioz Stars, 2026-04
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phosphor- (p-) akt (07-1398) antibody  (Merck KGaA)
90
Merck KGaA phosphor- (p-) akt (07-1398) antibody
The <t>phosphorylated-AKT1</t> <t>Thr308</t> level is relatively higher in Shock-Ens, while the expression of PLCβ3 is relatively higher in Mor-Ens. (A) Left, schematic of the experimental procedure for the in-situ proximity ligation assay. Right, the working principal diagram of PLA. (B, D) Representative images of PLA signaling for phosphor-AKT1 Thr308 or PLCβ3 in the VTA. Green: EGFP; Red: p-AKT1 or PLCβ3. Scale bar: left 100 μm, right 25 μm. Dashed lines: outline of EGFP + cell. (C, E) The violin plot and cumulative probability curves illustrate the distribution of the PLA signal intensity in each EGFP + cell. [Mann-Whitney test: (C) Left, Shock-Ens n = 822 cells from 5 mice, Mor-Ens n = 754 cells from 5 mice, U = 240371, P < 0.001; (E) left, Shock-Ens n = 1340 cells from 8 mice, Mor-Ens n = 1415 cells from 8 mice, U = 898116, P = 0.017. Kolmogorov-Smirnov test: (C) right, D = 0.179, P < 0.001; (E) right, D = 0.056, P = 0.026]. *P < 0.05, **P < 0.01, ***P < 0.001. Data are shown as mean ± SEM.
Phosphor (P ) Akt (07 1398) Antibody, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phosphor- (p-) akt (07-1398) antibody/product/Merck KGaA
Average 90 stars, based on 1 article reviews
phosphor- (p-) akt (07-1398) antibody - by Bioz Stars, 2026-04
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akt1 phosphor-mimetics  (Mimetics)
90
Mimetics akt1 phosphor-mimetics
The <t>phosphorylated-AKT1</t> <t>Thr308</t> level is relatively higher in Shock-Ens, while the expression of PLCβ3 is relatively higher in Mor-Ens. (A) Left, schematic of the experimental procedure for the in-situ proximity ligation assay. Right, the working principal diagram of PLA. (B, D) Representative images of PLA signaling for phosphor-AKT1 Thr308 or PLCβ3 in the VTA. Green: EGFP; Red: p-AKT1 or PLCβ3. Scale bar: left 100 μm, right 25 μm. Dashed lines: outline of EGFP + cell. (C, E) The violin plot and cumulative probability curves illustrate the distribution of the PLA signal intensity in each EGFP + cell. [Mann-Whitney test: (C) Left, Shock-Ens n = 822 cells from 5 mice, Mor-Ens n = 754 cells from 5 mice, U = 240371, P < 0.001; (E) left, Shock-Ens n = 1340 cells from 8 mice, Mor-Ens n = 1415 cells from 8 mice, U = 898116, P = 0.017. Kolmogorov-Smirnov test: (C) right, D = 0.179, P < 0.001; (E) right, D = 0.056, P = 0.026]. *P < 0.05, **P < 0.01, ***P < 0.001. Data are shown as mean ± SEM.
Akt1 Phosphor Mimetics, supplied by Mimetics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/akt1 phosphor-mimetics/product/Mimetics
Average 90 stars, based on 1 article reviews
akt1 phosphor-mimetics - by Bioz Stars, 2026-04
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phosphor-akt  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc phosphor-akt
(A). TSA induces <t>Akt</t> and GSK3β dephosphorylation in MCF-7 breast cancer cells. MCF-7 cells were incubated with 1 μM TSA for the indicated times. Following incubation, the cells were harvested and lysates were resolved by SDS-PAGE. Proteins were detected using the <t>indicated</t> <t>antibodies.</t> (B). The relative amounts of pAkt and pGSK3β in A were measured by densitometry and normalised to the amount of p38/SAPK. Result is representative of at least three separate experiments. (C). Knockdown of class I HDAC proteins induces Akt and GSK3β dephosphorylation. MCF-7 cells were transfected with oligo pools specifically targeting HDAC1, 2, 3 or a non-targeting siRNA pool (NSC). 72 h after transfection, cells were harvested and lysed. Lysates were treated as in A and probed with the indicated antibodies. (D). siRNA-mediated GSK3β knockdown attenuates the cytotoxic effect of TSA on MCF-7 cells. MCF-7 cells were transfected with oligo pools specifically targeting GSK3β or a non-targeting siRNA pool (NSC). 24 h after transfection cells were harvested and reseeded in 96-well plates and incubated for 24 h. Cells were then treated with 1 μM TSA for 48 h and relative cell survival was measured as described in materials and methods. Results represent the mean ± S.E. from at least three separate experiments. * P < 0.001 TSA treated vs. untreated NSC siRNA cells, ** P < 0.001 TSA treated NSC vs. TSA treated GSK3β siRNA cells. Inset: Lysates from cells transfected in parallel were probed with antibodies directed against GSK3 to monitor siRNA efficiency. (E). Effect of GSK3β siRNA on TSA induced cytotoxicity. Cells were treated as in D and examined by flow cytometry (see materials and methods section). Result is representative of at least three separate experiments. (F). Effect of GSK3β inhibition on TSA induced cytotoxicity. MCF-7 cells were cultured in 96-well plates with 10 -9 – 10 -5 M TSA alone or in combination with 10 mM LiCl. Relative cell survival was determined after 48 h as described in materials and methods section. Result is representative of three separate experiments.
Phosphor Akt, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phosphor-akt/product/Upstate Biotechnology Inc
Average 90 stars, based on 1 article reviews
phosphor-akt - by Bioz Stars, 2026-04
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phosphor-akt1 levels  (Consis Medical Ltd)
90
Consis Medical Ltd phosphor-akt1 levels
(A). TSA induces <t>Akt</t> and GSK3β dephosphorylation in MCF-7 breast cancer cells. MCF-7 cells were incubated with 1 μM TSA for the indicated times. Following incubation, the cells were harvested and lysates were resolved by SDS-PAGE. Proteins were detected using the <t>indicated</t> <t>antibodies.</t> (B). The relative amounts of pAkt and pGSK3β in A were measured by densitometry and normalised to the amount of p38/SAPK. Result is representative of at least three separate experiments. (C). Knockdown of class I HDAC proteins induces Akt and GSK3β dephosphorylation. MCF-7 cells were transfected with oligo pools specifically targeting HDAC1, 2, 3 or a non-targeting siRNA pool (NSC). 72 h after transfection, cells were harvested and lysed. Lysates were treated as in A and probed with the indicated antibodies. (D). siRNA-mediated GSK3β knockdown attenuates the cytotoxic effect of TSA on MCF-7 cells. MCF-7 cells were transfected with oligo pools specifically targeting GSK3β or a non-targeting siRNA pool (NSC). 24 h after transfection cells were harvested and reseeded in 96-well plates and incubated for 24 h. Cells were then treated with 1 μM TSA for 48 h and relative cell survival was measured as described in materials and methods. Results represent the mean ± S.E. from at least three separate experiments. * P < 0.001 TSA treated vs. untreated NSC siRNA cells, ** P < 0.001 TSA treated NSC vs. TSA treated GSK3β siRNA cells. Inset: Lysates from cells transfected in parallel were probed with antibodies directed against GSK3 to monitor siRNA efficiency. (E). Effect of GSK3β siRNA on TSA induced cytotoxicity. Cells were treated as in D and examined by flow cytometry (see materials and methods section). Result is representative of at least three separate experiments. (F). Effect of GSK3β inhibition on TSA induced cytotoxicity. MCF-7 cells were cultured in 96-well plates with 10 -9 – 10 -5 M TSA alone or in combination with 10 mM LiCl. Relative cell survival was determined after 48 h as described in materials and methods section. Result is representative of three separate experiments.
Phosphor Akt1 Levels, supplied by Consis Medical Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phosphor-akt1 levels/product/Consis Medical Ltd
Average 90 stars, based on 1 article reviews
phosphor-akt1 levels - by Bioz Stars, 2026-04
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akt (phospho thr308) antibody  (ImmunoWay Biotechnology Company)
90
ImmunoWay Biotechnology Company akt (phospho thr308) antibody
(A). TSA induces <t>Akt</t> and GSK3β dephosphorylation in MCF-7 breast cancer cells. MCF-7 cells were incubated with 1 μM TSA for the indicated times. Following incubation, the cells were harvested and lysates were resolved by SDS-PAGE. Proteins were detected using the <t>indicated</t> <t>antibodies.</t> (B). The relative amounts of pAkt and pGSK3β in A were measured by densitometry and normalised to the amount of p38/SAPK. Result is representative of at least three separate experiments. (C). Knockdown of class I HDAC proteins induces Akt and GSK3β dephosphorylation. MCF-7 cells were transfected with oligo pools specifically targeting HDAC1, 2, 3 or a non-targeting siRNA pool (NSC). 72 h after transfection, cells were harvested and lysed. Lysates were treated as in A and probed with the indicated antibodies. (D). siRNA-mediated GSK3β knockdown attenuates the cytotoxic effect of TSA on MCF-7 cells. MCF-7 cells were transfected with oligo pools specifically targeting GSK3β or a non-targeting siRNA pool (NSC). 24 h after transfection cells were harvested and reseeded in 96-well plates and incubated for 24 h. Cells were then treated with 1 μM TSA for 48 h and relative cell survival was measured as described in materials and methods. Results represent the mean ± S.E. from at least three separate experiments. * P < 0.001 TSA treated vs. untreated NSC siRNA cells, ** P < 0.001 TSA treated NSC vs. TSA treated GSK3β siRNA cells. Inset: Lysates from cells transfected in parallel were probed with antibodies directed against GSK3 to monitor siRNA efficiency. (E). Effect of GSK3β siRNA on TSA induced cytotoxicity. Cells were treated as in D and examined by flow cytometry (see materials and methods section). Result is representative of at least three separate experiments. (F). Effect of GSK3β inhibition on TSA induced cytotoxicity. MCF-7 cells were cultured in 96-well plates with 10 -9 – 10 -5 M TSA alone or in combination with 10 mM LiCl. Relative cell survival was determined after 48 h as described in materials and methods section. Result is representative of three separate experiments.
Akt (Phospho Thr308) Antibody, supplied by ImmunoWay Biotechnology Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/akt (phospho thr308) antibody/product/ImmunoWay Biotechnology Company
Average 90 stars, based on 1 article reviews
akt (phospho thr308) antibody - by Bioz Stars, 2026-04
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specific antibodies phosphor-akt  (Bioworld Antibodies)
90
Bioworld Antibodies specific antibodies phosphor-akt
(A). TSA induces <t>Akt</t> and GSK3β dephosphorylation in MCF-7 breast cancer cells. MCF-7 cells were incubated with 1 μM TSA for the indicated times. Following incubation, the cells were harvested and lysates were resolved by SDS-PAGE. Proteins were detected using the <t>indicated</t> <t>antibodies.</t> (B). The relative amounts of pAkt and pGSK3β in A were measured by densitometry and normalised to the amount of p38/SAPK. Result is representative of at least three separate experiments. (C). Knockdown of class I HDAC proteins induces Akt and GSK3β dephosphorylation. MCF-7 cells were transfected with oligo pools specifically targeting HDAC1, 2, 3 or a non-targeting siRNA pool (NSC). 72 h after transfection, cells were harvested and lysed. Lysates were treated as in A and probed with the indicated antibodies. (D). siRNA-mediated GSK3β knockdown attenuates the cytotoxic effect of TSA on MCF-7 cells. MCF-7 cells were transfected with oligo pools specifically targeting GSK3β or a non-targeting siRNA pool (NSC). 24 h after transfection cells were harvested and reseeded in 96-well plates and incubated for 24 h. Cells were then treated with 1 μM TSA for 48 h and relative cell survival was measured as described in materials and methods. Results represent the mean ± S.E. from at least three separate experiments. * P < 0.001 TSA treated vs. untreated NSC siRNA cells, ** P < 0.001 TSA treated NSC vs. TSA treated GSK3β siRNA cells. Inset: Lysates from cells transfected in parallel were probed with antibodies directed against GSK3 to monitor siRNA efficiency. (E). Effect of GSK3β siRNA on TSA induced cytotoxicity. Cells were treated as in D and examined by flow cytometry (see materials and methods section). Result is representative of at least three separate experiments. (F). Effect of GSK3β inhibition on TSA induced cytotoxicity. MCF-7 cells were cultured in 96-well plates with 10 -9 – 10 -5 M TSA alone or in combination with 10 mM LiCl. Relative cell survival was determined after 48 h as described in materials and methods section. Result is representative of three separate experiments.
Specific Antibodies Phosphor Akt, supplied by Bioworld Antibodies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/specific antibodies phosphor-akt/product/Bioworld Antibodies
Average 90 stars, based on 1 article reviews
specific antibodies phosphor-akt - by Bioz Stars, 2026-04
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anti-akt phosphor-specific pt308  (Biomol GmbH)
90
Biomol GmbH anti-akt phosphor-specific pt308
(A). TSA induces <t>Akt</t> and GSK3β dephosphorylation in MCF-7 breast cancer cells. MCF-7 cells were incubated with 1 μM TSA for the indicated times. Following incubation, the cells were harvested and lysates were resolved by SDS-PAGE. Proteins were detected using the <t>indicated</t> <t>antibodies.</t> (B). The relative amounts of pAkt and pGSK3β in A were measured by densitometry and normalised to the amount of p38/SAPK. Result is representative of at least three separate experiments. (C). Knockdown of class I HDAC proteins induces Akt and GSK3β dephosphorylation. MCF-7 cells were transfected with oligo pools specifically targeting HDAC1, 2, 3 or a non-targeting siRNA pool (NSC). 72 h after transfection, cells were harvested and lysed. Lysates were treated as in A and probed with the indicated antibodies. (D). siRNA-mediated GSK3β knockdown attenuates the cytotoxic effect of TSA on MCF-7 cells. MCF-7 cells were transfected with oligo pools specifically targeting GSK3β or a non-targeting siRNA pool (NSC). 24 h after transfection cells were harvested and reseeded in 96-well plates and incubated for 24 h. Cells were then treated with 1 μM TSA for 48 h and relative cell survival was measured as described in materials and methods. Results represent the mean ± S.E. from at least three separate experiments. * P < 0.001 TSA treated vs. untreated NSC siRNA cells, ** P < 0.001 TSA treated NSC vs. TSA treated GSK3β siRNA cells. Inset: Lysates from cells transfected in parallel were probed with antibodies directed against GSK3 to monitor siRNA efficiency. (E). Effect of GSK3β siRNA on TSA induced cytotoxicity. Cells were treated as in D and examined by flow cytometry (see materials and methods section). Result is representative of at least three separate experiments. (F). Effect of GSK3β inhibition on TSA induced cytotoxicity. MCF-7 cells were cultured in 96-well plates with 10 -9 – 10 -5 M TSA alone or in combination with 10 mM LiCl. Relative cell survival was determined after 48 h as described in materials and methods section. Result is representative of three separate experiments.
Anti Akt Phosphor Specific Pt308, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-akt phosphor-specific pt308/product/Biomol GmbH
Average 90 stars, based on 1 article reviews
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Image Search Results


The phosphorylated-AKT1 Thr308 level is relatively higher in Shock-Ens, while the expression of PLCβ3 is relatively higher in Mor-Ens. (A) Left, schematic of the experimental procedure for the in-situ proximity ligation assay. Right, the working principal diagram of PLA. (B, D) Representative images of PLA signaling for phosphor-AKT1 Thr308 or PLCβ3 in the VTA. Green: EGFP; Red: p-AKT1 or PLCβ3. Scale bar: left 100 μm, right 25 μm. Dashed lines: outline of EGFP + cell. (C, E) The violin plot and cumulative probability curves illustrate the distribution of the PLA signal intensity in each EGFP + cell. [Mann-Whitney test: (C) Left, Shock-Ens n = 822 cells from 5 mice, Mor-Ens n = 754 cells from 5 mice, U = 240371, P < 0.001; (E) left, Shock-Ens n = 1340 cells from 8 mice, Mor-Ens n = 1415 cells from 8 mice, U = 898116, P = 0.017. Kolmogorov-Smirnov test: (C) right, D = 0.179, P < 0.001; (E) right, D = 0.056, P = 0.026]. *P < 0.05, **P < 0.01, ***P < 0.001. Data are shown as mean ± SEM.

Journal: Theranostics

Article Title: Morphine- and foot shock-responsive neuronal ensembles in the VTA possess different connectivity and biased GPCR signaling pathway

doi: 10.7150/thno.90792

Figure Lengend Snippet: The phosphorylated-AKT1 Thr308 level is relatively higher in Shock-Ens, while the expression of PLCβ3 is relatively higher in Mor-Ens. (A) Left, schematic of the experimental procedure for the in-situ proximity ligation assay. Right, the working principal diagram of PLA. (B, D) Representative images of PLA signaling for phosphor-AKT1 Thr308 or PLCβ3 in the VTA. Green: EGFP; Red: p-AKT1 or PLCβ3. Scale bar: left 100 μm, right 25 μm. Dashed lines: outline of EGFP + cell. (C, E) The violin plot and cumulative probability curves illustrate the distribution of the PLA signal intensity in each EGFP + cell. [Mann-Whitney test: (C) Left, Shock-Ens n = 822 cells from 5 mice, Mor-Ens n = 754 cells from 5 mice, U = 240371, P < 0.001; (E) left, Shock-Ens n = 1340 cells from 8 mice, Mor-Ens n = 1415 cells from 8 mice, U = 898116, P = 0.017. Kolmogorov-Smirnov test: (C) right, D = 0.179, P < 0.001; (E) right, D = 0.056, P = 0.026]. *P < 0.05, **P < 0.01, ***P < 0.001. Data are shown as mean ± SEM.

Article Snippet: Primary antibodies: Anti-PLCβ3 Antibody (1:200, AP61288, Abcepta Biotech, USA), phosphor-AKT1 Thr308 antibody (1:200, AP67522, Abcepta Biotech, USA).

Techniques: Expressing, In Situ, Proximity Ligation Assay, MANN-WHITNEY

(A). TSA induces Akt and GSK3β dephosphorylation in MCF-7 breast cancer cells. MCF-7 cells were incubated with 1 μM TSA for the indicated times. Following incubation, the cells were harvested and lysates were resolved by SDS-PAGE. Proteins were detected using the indicated antibodies. (B). The relative amounts of pAkt and pGSK3β in A were measured by densitometry and normalised to the amount of p38/SAPK. Result is representative of at least three separate experiments. (C). Knockdown of class I HDAC proteins induces Akt and GSK3β dephosphorylation. MCF-7 cells were transfected with oligo pools specifically targeting HDAC1, 2, 3 or a non-targeting siRNA pool (NSC). 72 h after transfection, cells were harvested and lysed. Lysates were treated as in A and probed with the indicated antibodies. (D). siRNA-mediated GSK3β knockdown attenuates the cytotoxic effect of TSA on MCF-7 cells. MCF-7 cells were transfected with oligo pools specifically targeting GSK3β or a non-targeting siRNA pool (NSC). 24 h after transfection cells were harvested and reseeded in 96-well plates and incubated for 24 h. Cells were then treated with 1 μM TSA for 48 h and relative cell survival was measured as described in materials and methods. Results represent the mean ± S.E. from at least three separate experiments. * P < 0.001 TSA treated vs. untreated NSC siRNA cells, ** P < 0.001 TSA treated NSC vs. TSA treated GSK3β siRNA cells. Inset: Lysates from cells transfected in parallel were probed with antibodies directed against GSK3 to monitor siRNA efficiency. (E). Effect of GSK3β siRNA on TSA induced cytotoxicity. Cells were treated as in D and examined by flow cytometry (see materials and methods section). Result is representative of at least three separate experiments. (F). Effect of GSK3β inhibition on TSA induced cytotoxicity. MCF-7 cells were cultured in 96-well plates with 10 -9 – 10 -5 M TSA alone or in combination with 10 mM LiCl. Relative cell survival was determined after 48 h as described in materials and methods section. Result is representative of three separate experiments.

Journal: Molecular Cancer

Article Title: Role of glycogen synthase kinase 3 beta (GSK3β) in mediating the cytotoxic effects of the histone deacetylase inhibitor trichostatin A (TSA) in MCF-7 breast cancer cells

doi: 10.1186/1476-4598-5-40

Figure Lengend Snippet: (A). TSA induces Akt and GSK3β dephosphorylation in MCF-7 breast cancer cells. MCF-7 cells were incubated with 1 μM TSA for the indicated times. Following incubation, the cells were harvested and lysates were resolved by SDS-PAGE. Proteins were detected using the indicated antibodies. (B). The relative amounts of pAkt and pGSK3β in A were measured by densitometry and normalised to the amount of p38/SAPK. Result is representative of at least three separate experiments. (C). Knockdown of class I HDAC proteins induces Akt and GSK3β dephosphorylation. MCF-7 cells were transfected with oligo pools specifically targeting HDAC1, 2, 3 or a non-targeting siRNA pool (NSC). 72 h after transfection, cells were harvested and lysed. Lysates were treated as in A and probed with the indicated antibodies. (D). siRNA-mediated GSK3β knockdown attenuates the cytotoxic effect of TSA on MCF-7 cells. MCF-7 cells were transfected with oligo pools specifically targeting GSK3β or a non-targeting siRNA pool (NSC). 24 h after transfection cells were harvested and reseeded in 96-well plates and incubated for 24 h. Cells were then treated with 1 μM TSA for 48 h and relative cell survival was measured as described in materials and methods. Results represent the mean ± S.E. from at least three separate experiments. * P < 0.001 TSA treated vs. untreated NSC siRNA cells, ** P < 0.001 TSA treated NSC vs. TSA treated GSK3β siRNA cells. Inset: Lysates from cells transfected in parallel were probed with antibodies directed against GSK3 to monitor siRNA efficiency. (E). Effect of GSK3β siRNA on TSA induced cytotoxicity. Cells were treated as in D and examined by flow cytometry (see materials and methods section). Result is representative of at least three separate experiments. (F). Effect of GSK3β inhibition on TSA induced cytotoxicity. MCF-7 cells were cultured in 96-well plates with 10 -9 – 10 -5 M TSA alone or in combination with 10 mM LiCl. Relative cell survival was determined after 48 h as described in materials and methods section. Result is representative of three separate experiments.

Article Snippet: Antibodies to actin (Santa Cruz Biotechnology, Santa Cruz, CA), phosphor-Akt, Akt, phosphoGSK3β, GSK3β (Upstate Biotechnology, Dundee, United Kingdom), p38/SAPK (New England Biolabs, Hitchin, United Kingdom), and HDAC1, HDAC2, HDAC3 (Abcam, Cambridge, United Kingdom) were used.

Techniques: De-Phosphorylation Assay, Incubation, SDS Page, Transfection, Flow Cytometry, Inhibition, Cell Culture